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Nucleic Acids Research, 1988, Vol. 16, No. 22 10833-10847
© 1988

MOLECULAR BIOLOGY

A related moderately repetitive DNA family in the nematodes Ascaris lumbricoides and Panagrtllus silusiae

T. Warren and J.J. Pasternak*

Department of Biology, University of Waterloo, Waterloo Ontario N2L 3G1, Canada

*To whom correspondence should be addressed

Received October 13, 1987. Revised December 11, 1987. Accepted December 11, 1987.

Digestion of genomic DNA from the nematodes Panagrellus silusiae and Ascaris lumbricoides with restriction endonuclease BamH1 releases a 0.7 kilobase (kb) fragment. The 0.7 kb fragment from both nematodes was cloned onto E. coli plasmid pUC19. Using representative clones as DNA hybridization probes, it was found that (i) the BamHl fragments cross-hybridize; (ii) a ladder-effect with multiples of 0.7 kb was evident in both species after hybridization to genomic DNA and (iii) the genomic copy number of BamHl elements is 150 and 195 for P. silusiae and A. lumbricoides respectively. DNA sequence analysis of the inserts, AL700-1 and PS700-1, revealed nucleotide blocks with over 85% similarity. No open reading frames are present in either DNA fragment. Neither fragment hybridizes to genomic DNA from Caenorhabditis elegans. Northern blot hybridization indicated that the 0.7 kb element is transcribed into poly (A) -KNA in P. silusiae; but, is not transcribed in adult Ascaris muscle. Thus, P. silusiae and asdA. lumbricoides share a homologous, tandemly arrayed, moderately repetitive DNA family.


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