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Nucleic Acids Research, 1988, Vol. 16, No. 23 11027-11045
© 1988

MOLECULAR BIOLOGY

Erythroid specific activation of the Xenopus laevis adult {alpha}-globin promoter in transient heterokaryons

Jürg; Stalder

Zoologisches Institut, Abteilung für Zell- und Entwicklungsbiologie, Universität Bern CH-3012 Bern, Switzerland

Received September 22, 1988. Revised November 4, 1988. Accepted November 4, 1988.

Insertion of 1.5 kb of the 5'flanking region of the adult {alpha}-globin gene of X.laevis in front of the CAT structural gene promotes synthesis of CAT in transiently transfected X.laevis kidney cells. Fusion of transiently transfected kidney cells with erythroblasts isolated from anaemic frogs stimulates CAT expression 3–4 fold in the resulting transient heterokaryons. The stimulation is specific for the {alpha}-globin promoter and is obtained after fusion with erythroid cells but not with hepatocytes or kidney cells. Stably transfected kidney cells express drastically reduced CAT activity as compared with transiently transfected cells. Nevertheless, fusion of stably transfected kidney cells with erythroblasts leads to a 10–17 fold stimulation of CAT expression. The experiments suggest that erythroid specific transacting factors stimulate expression of CAT controlled by the adult {alpha}-globin promoter.


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