Skip Navigation

This Article
Right arrow Print PDF (1894K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Huang, Y.-q.
Right arrow Articles by Lane, M. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Huang, Y.-q.
Right arrow Articles by Lane, M. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 23 11125-11139
© 1988

ENZYMOLOGY

Actinomycin D induced DNase I cleavage enhancement caused by sequence specific propagation of an altered DNA structure

Yao-qi Huang, Robert P. Rehfuss, Steven R. LaPlante1, Ellis Boudreau1, Philip N. Borer1 and Michael J. Lane

Division of Hematology/Oncology, Departments of Medicine and Microbiology, State University of New York - Health Science Center at Syracuse Syracuse, NY 13210, USA 1Department of Chemistry and NIH Resource for NMR and Data Processing, Syracuse University Syracuse, NY 13244, USA

Received August 29, 1988. Revised November 3, 1988. Accepted November 3, 1988.

Two DNA hexadecamers containing one central 5'-GC-3' base step have been examined by footprinting methodology in the presence and absence of actinomycin D. The results of these studies, coupled with imino proton NMR measurements indicate that the antitumor drug causes a change in DNA conformation at a distance from the actinomycin intercalation site in a molecule of sequence d[ATATATAGCTATATAT] that does not occur in d[AAAAAAAGCTTTTTTT] The experiments demonstrate that DNase I rate enhancements associated with actinomycin D binding are caused by ligand alteration of equilibrium DNA structure.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.