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Nucleic Acids Research, 1988, Vol. 16, No. 23 11187-11205
© 1988

MOLECULAR BIOLOGY

The structure of nucleosomal core particles within transcribed and repressed gene regions

Vasilyi M. Studitsky, Alexander V. Belyavsky, Anna F. Melnikova and Andrei D. Mirzabekov

Institute of Molecular Biology, USSR Academy of Sciences Moscow 117984, USSR

Received July 11, 1988. Revised November 3, 1988. Accepted November 3, 1988.

The arrangement of histones along DNA in nucleosomal core particles within transcribed heat shock gene (hsp 70) region and repressed insertion within ribosomal genes of Drosophila was analysed by using protein-DNA crosslinking methods combined with hybridization tests In addition, two-dimensional gel electrophoresis was employed to compare the overall nucleosomal shape and the nucleosomal DNA size. The arrangement of histones along DNA and general compactness of nucleosomes were shown to be rather similar in transcriptionally active and inactive genomic regions. On the other hand, nucleosomes with in transcriptionally active chromatin are characterized by a larger size of nucleosomal DNA produced by micrococcal nuclease digestion and some peculiarity in electrophoretic mobility.


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