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Nucleic Acids Research, 1988, Vol. 16, No. 24 11461-11467
© 1988

MOLECULAR BIOLOGY

Rapid physical mapping of the Mycoplasma mobile genome by two-dimensional field inversion gel electrophoresis techniques

Wilfried Bautsch

Zentrum Biochemie, Abteilung Biophysikalische Chemie, Medizinische Hochschule Hannover D-3000 Hannover 61, FRG

Received October 10, 1988. Revised November 15, 1988. Accepted November 15, 1988.

A macrorestriction map of the 780 kbp Mycoplasma mobile (ATCC 43663) genome was constructed. Linking fragments were identified on two-dimensional pulsed-field electrophoresis gels. Either complete double restriction digests or partial and complete single digests were separated in the first and second dimension, respectively. 19 restriction sites of four enzymes could be assigned to the map. These rapid methods do not require DNA probes and are applicable to the long-range restriction mapping of all genomes that yield resolvable patterns on two-dimensional gels.


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