Skip Navigation

This Article
Right arrow Print PDF (5178K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Planck, S. R.
Right arrow Articles by Buckley, S. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Planck, S. R.
Right arrow Articles by Buckley, S. D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 24 11663-11673
© 1988

MOLECULAR BIOLOGY

Modulation of hnRNP A1 protein gene expression by epidermal growth factor in Rat-1 cells

Stephen R. Planck, Mark D. Listerud+ and Sharon D. Buckley§

Department of Medical Biochemistry and Anatomy, University of Calgary Calgary, Alberta, Canada

Present addresses: +Anatomy and Cell Biology, Columbia University, New York, NY 10032

Present addresses: §Microbiology and Immunology, Oregon Health Sciences University, Portland, OR 97201, USA

Received June 24, 1988. Revised November 13, 1988. Accepted November 13, 1988.

The levels of mRNA encoding hnRNP core protein A1 have been compared in quiescent and proliferating Rat-1 embryonic fibroblasts. Northern blot hybridization analyses using probes made from an A1 cDNA clone, {lambda}HDP-182, isolated by Cobianchi et al. (J. Biol. Chem. 261:3536-2543 (1986)) indicated that three sizes of poly A+ RNAs, 1.6, 2.0, & 4.0 kb, have extensive sequence homology. The levels of all three A1 RNA species are responsive to the proliferation state of the cells. Stimulation of quiescent Rat-1 cells with serum or epidermal growth factor resulted in a 2- to 5-fold increase in the levels of each of these three RNAs that was evident after 2 hours and reached a peak after about 8 hours. Addition of the protein synthesis inhibitor, cycloheximide, along with epidermal growth factor completely blocked the upsurge in A1 RNA levels. Thus, the A1 RNA species are not primary transcriptional targets of epidermal growth factor but do show an induction pattern similar to mRNAs encoding some glycolytic enzymes.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
A. Audibert, D. Weil, and F. Dautry
In Vivo Kinetics of mRNA Splicing and Transport in Mammalian Cells
Mol. Cell. Biol., October 1, 2002; 22(19): 6706 - 6718.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
F. Raffalli-Mathieu, T. Glisovic, Y. Ben-David, and M. A. Lang
Heterogeneous Nuclear Ribonucleoprotein A1 and Regulation of the Xenobiotic-Inducible Gene Cyp2a5
Mol. Pharmacol., April 1, 2002; 61(4): 795 - 799.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
B. C. Blaxall, A. C. Pellett, S. C. Wu, A. Pende, and J. D. Port
Purification and Characterization of beta -Adrenergic Receptor mRNA-binding Proteins
J. Biol. Chem., February 11, 2000; 275(6): 4290 - 4297.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. Zhou, J. L. Mulshine, E. J. Unsworth, F. M. Scott, I. M. Avis, M. D. Vos, and A. M. Treston
Purification and Characterization of a Protein That Permits Early Detection of Lung Cancer
J. Biol. Chem., May 3, 1996; 271(18): 10760 - 10766.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.