Skip Navigation

This Article
Right arrow Print PDF (511K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (56)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hatch, C. L.
Right arrow Articles by Bonner, W. M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hatch, C. L.
Right arrow Articles by Bonner, W. M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 3 1113-1124
© 1988

Articles

Sequence of cDNAs for mammalian H2A.Z, an evolutionarily diverged but highly conserved basal histone H2A isoprotein species

Christopher L. Hatch and William M. Bonner*

Laboratory of Molecular Pharmacology, Division of Cancer Treatment, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received November 1, 1987. Revised January 6, 1988. Accepted January 6, 1988.

The nucleotide sequences of cDNAs for the evolutionarily diverged but highly conserved basal H2A isoprotein, H2A.Z, have been determined for the rat, cow, and human. As a basal histone, H2A.Z is synthesized throughout the cell cycle at a constant rate, unlinked to DNA replication, and at a much lower rate in quiescent cells. Each of the cDNA isolates encodes the entire H2A.Z polypeptide. The human isolate is about 1.0 kilobases long. It contains a coding region of 387 nucleotides flanked by 106 nucleotides of 5'UTR and 376 nucleotides of 3'UTR, which contains a polyadenylation signal followed by a poly A tail. The bovine and rat cDNAs have 97 and 94% nucleotide positional identity to the human cDNA in the coding region and 98% in the proximal 376 nucleotides of the 3'UTR which includes the polyadenylation signal. A potential stem-forming sequence imbedded in a direct repeat is found centered at 261 nucleotides into the 3'UTR. Each of the cDNA clones could be transcribed and translated in vitro to yield H2A.Z protein. The mammalian H2A.Z cDNA coding sequences are approximately 80% similar to those in chicken and 75% to those in sea urchin.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
D. W. Abbott, V. S. Ivanova, X. Wang, W. M. Bonner, and J. Ausio
Characterization of the Stability and Folding of H2A.Z Chromatin Particles. IMPLICATIONS FOR TRANSCRIPTIONAL ACTIVATION
J. Biol. Chem., November 2, 2001; 276(45): 41945 - 41949.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
B. P. Chadwick and H. F. Willard
Histone H2A variants and the inactive X chromosome: identification of a second macroH2A variant
Hum. Mol. Genet., May 1, 2001; 10(10): 1101 - 1113.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
B. P. Chadwick and H. F. Willard
A Novel Chromatin Protein, Distantly Related to Histone H2A, Is Largely Excluded from the Inactive X Chromosome
J. Cell Biol., January 22, 2001; 152(2): 375 - 384.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
L A Stargell, J Bowen, C A Dadd, P C Dedon, M Davis, R G Cook, C D Allis, and M A Gorovsky
Temporal and spatial association of histone H2A variant hv1 with transcriptionally competent chromatin during nuclear development in Tetrahymena thermophila.
Genes & Dev., December 1, 1993; 7(12b): 2641 - 2651.
[Abstract] [PDF]

Home page
 ScienceHome page
Pehrson JR and V. Fried
MacroH2A, a core histone containing a large nonhistone region
Science, September 4, 1992; 257(5075): 1398 - 1400.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.