Nucleic Acids Research, 1988, Vol. 16, No. 3 1135-1141
© 1988
Articles |
The mechanisms of action of E.coli endonuclease III and T4 UV endonuclease (endonuclease V) at AP sites
Department of Biochemistry, University of California Berkeley, CA 94720, USA
Received October 13, 1987. Revised January 6, 1988. Accepted January 6, 1988.
Treatment of DNA containing AP sites with either T4 UV endonuclease or with E. coli endonuclease III followed by a human class II AP endonuclease releases a putative ß-elimination product. This result suggests that both the T4 endonuclease and E. coli endonuclease III class I AP endonucleases catalyze pbosphodiester bond cleavage via a lyase- rathar than a hydrolase mechanism. Indeed, we have not detected a class I AP endonuclease which hydrolytically catalyzes phosphodiester bond cleavage. Whereas these enzymes use a lyase-like rather than a hydrolytic mechanism, they nonetheless catalyze phosphodiester bond cleavage. We suggest that the term endonuclease can be properly applied to them.
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