Skip Navigation

This Article
Right arrow Print PDF (2451K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Braaten, D. C.
Right arrow Articles by D'Urso, M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Braaten, D. C.
Right arrow Articles by D'Urso, M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 3 865-881
© 1988

Articles

Locations and contexts of sequences that hybridize to poly(dG-dT).(dC-dA) in mammalian ribosomal DNAs and two X-linked genes

Douglas C. Braaten, James R. Thomas, Randall D. Little, Kevin R. Dickson, Ilya Goldberg, David Schlessinger, Alfredo Ciccodicola1 and Michele D'Urso

Department of Microbiology and Immunology, Washington University School of Medicine St Louis, MO 63110, USA 1International Institute of Genetics and Biophysics Naples 80125, Italy

Received October 29, 1987. Accepted December 21, 1987.

Sequences located several kilobases both 5' and 3' of the stably transcribed portion of several genes hybridize to radio-labeled pure fragments of the alternating sequence poly (dG-dT)(dC-dA) [poly(GT)]. The genes include the ribosornal DNA of mouse, rat, and human, and also human glucose-6-phosphate dehydrogenase (G6PD) and mouse hypoxanthine-guanine phosphoribosyl transferase (HPRT). HPRT has additional hybridizing sequences in introns. Fragments that include the hybridizing sequences and up to 300 bp of adjoining DNA show perfect runs of poly(GT) (>30bp) in all but the human 5' region of rDNA, which shows a somewhat different alternating purine:pyrimidine sequence, poly(GTAT) (36bp). Within 150 bp of these sequences in various instances are found a number of other sequences reported to affect DNA conformation in model systems. Most marked is an enhancement of sequences matching at least 67% to the consensus binding sequence for topoisomerase II. Two to ten-fold less of such sequences were found in other sequenced portions of the nontranscribed spacer or in the transcribed portion of rDNA. The conservation of the locations of tracts of alternating purlne:pyrimidine between evolutionarily diverse species is consistent with a possible functional role for these sequences.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Cancer Res.Home page
D. G. Ginzinger, T. E. Godfrey, J. Nigro, D. H. Moore II, S. Suzuki, M. G. Pallavicini, J. W. Gray, and R. H. Jensen
Measurement of DNA Copy Number at Microsatellite Loci Using Quantitative PCR Analysis
Cancer Res., October 1, 2000; 60(19): 5405 - 5409.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.