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Nucleic Acids Research, 1988, Vol. 16, No. 3 895-905
© 1988

Articles

In vitro transcribed SV4O minichromosomes, as the bulk mlnichromosomes, have a low level of unconstrained negative supercoils

Mordechai Choder and Yosef Aloni

Department of Genetics, Weizmann institute of Science Rehovot 76100, Israel

Received October 22, 1987. Accepted December 29, 1987.

The method for detecting the small fraction (1%) of transcriptionally active SV40 ininichromosomes, in the presence of the bulk minichromosornes, (14) has been applied to directly analyze the topology of transcribed and non-transcribed minichrornosomal DNA. We show here that DNA of both transcribed and non-transcribed minichromosomes have the same number of supercoils which are constrained by nucleosomes. In addition, minichromosomal DNA of both fractions have a low level of unconstrained supercoils (1–2 extra supercoils) which can be relaxed in vitro by topoisornerase I.


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