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Nucleic Acids Research, 1988, Vol. 16, No. 4 1319-1331
© 1988

Articles

Transcription of human 7S K DNA in vitro and in vivo is exclusively controlled by an upstream promoter

Hartmut Kleinert and Bernd-Joachim Benecke*

Lehrstuhl für Biochemie, Ruhr-Universität D-463 Bochum 1, FRG

*to whom correspondence should be addressed

Received November 23, 1987. Revised January 19, 1988. Accepted January 19, 1988.

We have analyzed the transcription of a recently isolated human 7S K RNA gene in vitro and in vivo. In contrast to hitherto characterized class III gens (genes transcribed by RNA polymerase III), the coding sequence of this gene is not required for faithful and efficient transcription by RNA polymerase III. In fact, a procaryotic vector DNA sequence was efficiently transcribed by RNA polymerase III under the control of the 7S K RNA gene upstream sequence in vitro and in vivo. S1 nuclease protection analyses confirmed that the 7S K 5'flanking sequence was sufficient for accurate transcription initiation. These data demonstrate that 7S K DNA represents a novel class III gene. the promoter elements of which are located outside the coding sequence.


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