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Nucleic Acids Research, 1988, Vol. 16, No. 4 1423-1430
© 1988

Articles

Gene transactivation mediated by the TAT gene of human immunodeficiency virus in transgenic mice

Jaspal S. Khillan, Keith C. Deen1, Shu-hua Yu, Raymond W. Sweet1, Martin Rosenberg and Heiner Westphal*

Laboratory of Molecular Genetics, National Institute of Child Health and Human Development Bethesda, MD 20892, USA 1Department of Molecular Genetics, Smith Kline and French Laboratories Philadelphia, PA 19101, USA

*To whom correspondence should be addressed

Received October 5, 1987. Revised January 20, 1988. Accepted January 20, 1988.

Transgenic mice were generated carrying either the long terminal repeat of Human Immunodeficiency Virus fused to the bacterial chloramphenicol acetyl transferase reporter gene or a control element of the murine {alpha}A crystallin gene fused to the tat gene of human immunodeficiency virus. By crossing these two strains, progeny were obtained which carried both transgenes. The bacterial reporter gene was specifically transactivated in the eyes of these animals.


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