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Nucleic Acids Research, 1988, Vol. 16, No. 4 1593-1601
© 1988

Articles

The genetic structure of mouse ornithine transcarbamylase

Steven E. Scherer, Gabor Veres* and C.Thomas Caskey

Institute for Molecular Genetics, Howard Hughes Medical Institute, Baylor College of Medicine 1 Baylor Plaza, Houston, TX 77030, USA

Received November 3, 1987. Accepted December 14, 1987.

The gene encoding the mouse urea cycle enzyme, ornithine transcarbamylase has been isolated on five partially overlapping bacterlophage lambda clones. We have characterized the gene and found that it is split between ten exons distributed over approximately 70 kb of the X chromosone. The introns range in size from 88 bases to the relatively unusual size of approximately 26 kilobases, while the splice donor/splice acceptor sequences conform to the consensus established for other eukaryotic genes.

*Permanent address: Hungarian Academy of Science, Biological Research Center, 6701 Szeged, PO Box 521, Hungary


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