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Nucleic Acids Research, 1988, Vol. 16, No. 5 1693-1702
© 1988

Articles

The design of syntetic genes

Scott R. Presnell and Steven A. Benner

Laboratory for Organic Chemistry, ETH-Zentrum, Universtätstrasse 16 CH-8092 Zurich, Switzerland

Received August 17, 1987. Revised December 9, 1987. Accepted December 9, 1987.

Computer programs are described that aid in the design of synthetic genes coding for proteins that are targets of a research program in site directed mutagenesis. These programs "reverse-translate" protein sequences into general nucleic acid sequences (those where codons have not yet been selected), map restriction sites into general DNA sequences, identify points in the synthetic gene where unique restriction sites can be introduced, and assist in the design of genes coding for hybrids and evolutionary intermediates between homologous pro teins. Application of these programs therefore facilitates the use of modular mutagenesis to create variants of proteins, and the implementation of evolutionary guidance as a strategy for selecting mutants.


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K. Sivaraman, A. Seshasayee, P. M. Tarwater, and A. M. Cole
Codon choice in genes depends on flanking sequence information--implications for theoretical reverse translation
Nucleic Acids Res., February 11, 2008; 36(3): e16 - e16.
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