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Nucleic Acids Research, 1988, Vol. 16, No. 5 1759-1766
© 1988

Articles

Regen: program for designing gene assembly

Roman Jerala and Vito Turk

Department of Biochemistry, Jozf Stefan Institute Jamova 39, 61000 Ljubljana, Yugoslavia

Received September 9, 1987. Revised February 15, 1988. Accepted February 15, 1988.

One of the main problems in constructing sythetic genes is the incorrect hybridisation between the oligonucleotides. The problem is resolved if the sequence uniquely defines the position of the oligonucleotide in the assembled gene. This can be accomplished through the wise partition of d5DNA sequence in the fragments. We describe a program for use in designing such gene assembly. For a given DNA sequence and the approximate location of oligonucleotide boundary it generates all sets of protruding ends that share the smallest homology.


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