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Nucleic Acids Research, 1988, Vol. 16, No. 5 1779-1787
© 1988

Articles

Computer simulation of DNA ligation: determination of initial DNA concentrations favouring the formation of recombinant molecules

Frédéric Dardel

Laboratoire de Biochimie, Ecole Polytechnique UA no. 240 du CNRS, 91128 Palaiseau Cedex, France

Received September 18, 1987. Revised December 15, 1987. Accepted December 15, 1987.

A program is described which aligns pairs of linear restriction site maps using an algorithm which maximizes the number of coincident sites through the addition of extra ‘padding’ fragments; and makes maps of the non-conserved sites between pairs and triplets of input maps. A range of display facilities, hardcopy functions and database management routines are also included. The program runs on a stand-alone microcomputer, and is stored along with a database of several hundred files on a single floppy disc.


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