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Nucleic Acids Research, 1988, Vol. 16, No. 5 1801-1812
© 1988


Articles

Electronic fingerprinting of RNA

Peter Gegenheimer

Departments of Biochemistry and Botany, and Molecular Genetics Program, University of Kansas Lawrence, KS 66045, USA

Received August 17, 1987. Revised December 31, 1987. Accepted December 31, 1987.

We have developed a computer graphics program system for the schematic representation of several protein secondary structure analysis algorithms. The programs calculate the probability of occurrence of {alpha}-helix, ß-sheet and ß-turns by the method of Chou and Fasman and assign unique predicted structure to each residue using a novel conflict resolution algorithm based on maximum liklihood. A detailed structure map containing secondary structure, hydrophobicity, sequence identity, sequence numbering and the location of putative N-linked glycosylation sites is then produced. In addition, helical wheel diagrams and hydrophobic moment calculations can be performed to further analyze the properties of selected regions of the sequence. As they require only structure specification as input, the graphics programs can easily be adapted for use with other secondary structure prediction schemes. The use of these programs to analyze protein structure-function relationships is described and evaluated.


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