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Nucleic Acids Research, 1988, Vol. 16, No. 5 1847-1856
© 1988

Articles

Automated sequence reading and analysis

John West

Bio-Rad Laboratories 3316 Spring Garden Street, Philadelphia, PA 19104, USA

Received August 17, 1987. Revised December 31, 1987. Accepted December 31, 1987.

We report on a system developed by Bio-Rad Laboratories, INC. which combines automated reading of DNA sequencing autoradiograms with comprehensive software for shotgun overlapping of the readings, analysis of the sequences derived and searching of databases. Reading is accomplished using a high speed optical scanner and pattern recognition software operating on a personal computer. Overlapping, analysis and database searching software each incorporate significant advances over prior systems.


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