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Nucleic Acids Research, 1988, Vol. 16, No. 5 2015-2030
© 1988

Articles

The interaction of Eschenchia coli integration host factor with the cohesive end sites of phages {lambda} and 21

Winnie Xin and Michael Feiss

Department of Microbiology, College of Medicine, and Interdepartmental Genetics Ph.D. Program, University of Iowa Iowa City, IA 52242, USA

Received November 30, 1987. Revised January 25, 1988. Accepted January 25, 1988.

The interaction of E. coli integration host factor (IHF) with the cohesive end sites (cos's) of phages {lambda} and 21 has been studied by the DNAase I footprinting technique. Six potential sites in cos {lambda} differ from the consensus IHF binding sequence by 1 to 3 base pairs. Of the six, one site, I1, binds IHF strongly. The I1 segment protected by IHF contains two sequences that closely match the IHF consensus binding sequence. Another site, I2, binds IHF moderately well, and three sites: I0', I3 and I4 bind IHF very weakly. The I0 site does not bind IHF under the conditions used here. In phage 21 the DNA segment extending to the right from the cohesive ends, which contains three potential IHF binding sites, was examined. Two sites bind IHF well; I1, the 21 analogue of one of the {lambda} I1 sites, and I0, a site not analogous to a {lambda} site. The third 21 site, I2, binds IHF moderately well, as does the analogous I2 site in {lambda}. The significance of the results for {lambda} DNA packaging is discussed.


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