Nucleic Acids Research, 1988, Vol. 16, No. 5 2127-2148
© 1988
Articles |
Nucleotide sequence determination and secondary structure of Xenopus U3 snRNA
Brown University, Division of Biology and Medicine Providence, RI 02912, USA
+To whom correspondence should be addressed
Received September 28, 1987. Revised January 13, 1988. Accepted January 13, 1988.
Using a combination of RNA sequencing and construction of cDNA clones followed by DNA sequencing, we have determined the primary nucleotide sequence of U3 snRNA in Xenopus laevis and Xenopus borealis This molecule has a length of 219 nucleotides. Alignment of the Xenopus sequences with U3 snRNA sequences from other organisms reveals three evolutionarily conserved blocks. We have probed the secondary structure of U3 snRNA in intact Xenopus laevis nuclei using single-strand specific chemical reagents; primer extension was used to map the positions of chemical modification. The three blocks of conserved sequences fall within single-stranded regions, and are therefore accessible for interaction with other molecules. Models of U3 snRNA function are discussed in light of these data.
*Present address: Panum Institute, Department of Biochemistry B, Blegdamsvej 3C, University of Copenhagen, DK-2200 Copenhagen N., Denmark
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