Skip Navigation

This Article
Right arrow Print PDF (6326K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (34)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Arnold, H.H.
Right arrow Articles by Paterson, B.M.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Arnold, H.H.
Right arrow Articles by Paterson, B.M.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 6 2411-2429
© 1988

Articles

The promoter of the chicken cardiac myosin light chain 2 gene shows cell-specific expression in transfected primary cultures of chicken muscle

H.H. Arnold*, E. Tannich and B.M. Paterson

Department of Toxicology, Medical School, University of Hamburg Grindelallee 117, D-2000

*To whom correspondance should be address

Received January 26, 1988. Accepted February 25, 1988.

Transcriptional regulation of the chicken cardiac myosin light chain 2 (MLC2-A) gene was investigated in chicken primary myoblast and fibroblast cultures transfected with vector constructs containing the bacterial marker gene for chloramphenicol acetyl-transferase (CAT) under the control of the MLC2-A promoter. We here demonstrate that sequences close to the TATA box are sufficient to direct muscle specific and regulated expression of the MLC2-A mRNA. Transcription from MLC2-A promoter/CAT hybrids in myocytes starts from the authentic cap site that is also used in vivo. In primary breast muscle cells, bromodeoxyuridine (BUdR), a reversible blocking agent of cell differentiation, suppresses transcription from the MLC2-A promoter whereas nonmuscle promoters like the RSV- or the cytoplasmic ß-actin promoter are unaffected in their transcriptional capacity. Although the endogenous cardiac MLC2-A gene in chicken is exclusively active in heart, the transfected MLC 2-A promoter escapes this cell type control in primary cultures of breast muscle. These results demonstrate that although muscle specificity of the MLC2-A gene and its transcriptional up-regulation during diffentiation is maintained in a rather short promoter segment, restrictive elements determining the muscle cell type specificity in vivo are either not present in our constructs or are not acting under the conditions of transient transfection.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
S. Mathew, E. Mascareno, and M. A. Q. Siddiqui
A Ternary Complex of Transcription Factors, Nished and NFATc4, and Co-activator p300 Bound to an Intronic Sequence, Intronic Regulatory Element, Is Pivotal for the Up-regulation of Myosin Light Chain-2v Gene in Cardiac Hypertrophy
J. Biol. Chem., September 24, 2004; 279(39): 41018 - 41027.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.