Skip Navigation

This Article
Right arrow Print PDF (1769K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Stunnenberg, H. G.
Right arrow Articles by van der Vlietl, P. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Stunnenberg, H. G.
Right arrow Articles by van der Vlietl, P. C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 6 2431-2444
© 1988

Articles

High expression of functional adenovirus DNA polymerase and precursor terminal protein using recombinant vaccinia virus

Henk G. Stunnenberg, Heidrun Lange, Lennart Philipson, Rob T. van Miltenburg1 and Peter C. van der Vlietl*

European Molecular Biology Laboratory Meyerhofstrasse 1, 6900 Heidelberg FRG 1Laboratory for Physiological Chemistry, State University of Utrecht Vondellaan 24a, 3521 GG Utrecht, The Netherlands

*To whom correspondance should be address

Received January 25, 1988. Revised February 26, 1988. Accepted February 26, 1988.

Initiation of Adenovirus (Ad) DNA replication occurs by a protein-priming mechanism in which the viral precursor terminal protein (pTP) and DNA polymerase (pol) as well as two nuclear DNA-binding proteins from uninfected HeLa cells are required. Biochemical studies on the pTP and DNA polymerase proteins separately have been hampered due to their low abundance and their presence as a pTP-pol complex in Ad infected cells. We have constructed a genomic sequence containing the large open reading frame from the Ad5 pol gene to which 9 basepairs from a putative exon were ligated. When inserted behind a modified late promoter of vaccinia virus the resulting recombinant virus produced enzymaticalty active 140 kDa Ad DNA polymerase. The same strategy was applied to express the 80 kDa pTP gene in a functional form. Both proteins were overexpressed at least 30-fold compared to extracts from Adenovirus infected cells and, when combined, were fully active for initiation in an in vitro Adenovirus DNA replication system.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Virol.Home page
G. Regl, A. Kaser, M. Iwersen, H. Schmid, G. Kohla, B. Strobl, U. Vilas, R. Schauer, and R. Vlasak
The Hemagglutinin-Esterase of Mouse Hepatitis Virus Strain S Is a Sialate-4-O-Acetylesterase
J. Virol., June 1, 1999; 73(6): 4721 - 4727.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
G. Lepperdinger, B. Strobl, and G. Kreil
HYAL2, a Human Gene Expressed in Many Cells, Encodes a Lysosomal Hyaluronidase with a Novel Type of Specificity
J. Biol. Chem., August 28, 1998; 273(35): 22466 - 22470.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. A. Kummer, A. M. Kamp, F. Citarella, A. J. G. Horrevoets, and C. E. Hack
Expression of Human Recombinant Granzyme A Zymogen and Its Activation by the Cysteine Proteinase Cathepsin C
J. Biol. Chem., April 19, 1996; 271(16): 9281 - 9286.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. G. Horrevoets, A. E. Smilde, J. C. Fredenburgh, H. Pannekoek, and M. E. Nesheim
The Activation-resistant Conformation of Recombinant Human Plasminogen Is Stabilized by Basic Residues in the Amino-terminal Hinge Region
J. Biol. Chem., June 30, 1995; 270(26): 15770 - 15776.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Houhou, A. Lamouroux, N. F. Biguet, and J. Mallet
Expression of Human Dopamine [IMAGE]-Hydroxylase in Mammalian Cells Infected by Recombinant Vaccinia Virus
J. Biol. Chem., May 26, 1995; 270(21): 12601 - 12606.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R Valcarcel, H Holz, C G Jimenez, D Barettino, and H G Stunnenberg
Retinoid-dependent in vitro transcription mediated by the RXR/RAR heterodimer.
Genes & Dev., December 15, 1994; 8(24): 3068 - 3079.
[Abstract] [PDF]

Home page
 J. Cell Sci.Home page
J Wang, M Satoh, A Pierani, J Schmitt, C. Chou, H. Stunnenberg, R. Roeder, and W. Reeves
Assembly and DNA binding of recombinant Ku (p70/p80) autoantigen defined by a novel monoclonal antibody specific for p70/p80 heterodimers
J. Cell Sci., January 11, 1994; 107(11): 3223 - 3233.
[Abstract] [PDF]

Home page
 Genes Dev.Home page
C Disela, C Glineur, T Bugge, J Sap, G Stengl, J Dodgson, H Stunnenberg, H Beug, and M Zenke
v-erbA overexpression is required to extinguish c-erbA function in erythroid cell differentiation and regulation of the erbA target gene CAII.
Genes & Dev., November 1, 1991; 5(11): 2033 - 2047.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. B. Brenkman, M. R. Heideman, V. Truniger, M. Salas, and P. C. van der Vliet
The (I/Y)XGG Motif of Adenovirus DNA Polymerase Affects Template DNA Binding and the Transition from Initiation to Elongation
J. Biol. Chem., August 3, 2001; 276(32): 29846 - 29853.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.