Skip Navigation

This Article
Right arrow Print PDF (2800K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Leontis, N.
Right arrow Articles by Engelke, D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Leontis, N.
Right arrow Articles by Engelke, D.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 6 2537-2552
© 1988

Articles

Effects of tRNA-intron structure on cleavage of precursor tRNAs by RNase P from Saccharomyces cerevisiae

Neocles Leontis+,*, Andrew DaLio*, Marjorie Strobel1 and David Engelke

Department of Biological Chemistry, University of Michigan Ann Arbor, Ml 48109, USA 1LBI Basic Research Program, NCI-FCRF PO Box B, Frederick, MD 21701, USA

*The first author contributed equally to this work

Received November 6, 1987. Revised February 17, 1988. Accepted February 17, 1988.

RNase P derived from S.cerevisiae nuclei was tested for its ability to cleave a variety of naturally occurring and selectively altered precursor-tRNA molecules to yield matured 5' termini. Precursors were synthesized in vitro in order to test which aspects of substrate structure are crucial to recognition and cleavage by RNase P. Base modifications in the precursor substrates are not required for cleavage by the enzyme, but deletion and substitution mutations affecting any portion of the precursor tertiary structure reduce cleavage. In particular, a number of alterations in the intervening sequence (IVS) reduce the susceptibility of the substrate to cleavage by RNase P. The significance of these results is discussed in reference to the contribution of the IVS to the structure of the precursor-tRNA.

+Present Address: Chemistry Department, Bowling Green State University, Bowling Green, OH 43403, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Genes Dev.Home page
E. Bertrand, F. Houser-Scott, A. Kendall, R. H. Singer, and D. R. Engelke
Nucleolar localization of early tRNA processing
Genes & Dev., August 15, 1998; 12(16): 2463 - 2468.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.