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Nucleic Acids Research, 1988, Vol. 16, No. 6 2585-2599
© 1988

Articles

Sequences between the internal control regions of tRNAArg of Drosophila melanogasrer influence stimulation of transcription of the 5' flanking DNA

Daniel Horvath and George B. Spiegelman*

Departments of Medical Genetics and Microbiology, University of British Columbia Vancouver, British Columbia V6T 1W5, Canada

*To whom correspondance should be addressed

Received June 19, 1987. Revised February 24, 1988. Accepted February 24, 1988.

Recombinants between 5' deletion mutants of a tRNAVal3b gene which is inactive as an in vitro transcription template and a CRNAArg gene, which is an active in vitro template were made. The 5' flanking region of tRNAArg including 36 nucleotides of the coding sequence of the gene stimulated transcription of the tRNAVal3b, deleted to the +17 position, gene by over 50 fold. When the 5' flanking region of the tRNArg gene included 22 nucleotides of the coding sequence stimulation was reduced by a factor of 3. Thus the sequences between +22 and +36 of tRNAArg are required to permit maximum stimulation of tRNAVal3b in vitro template activity.


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