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Nucleic Acids Research, 1988, Vol. 16, No. 6 2601-2612
© 1988

Articles

Randomly picked cosmid clones overlap the pyrB and oriC gap in the physical map of the E.coli chromosome

V. Knott, D.J.G. Rees*, Z. Cheng+ and G.G. Brownlee

Sir William Dunn School of Pathology, University of Oxford South Parks Road, Oxford OX1 3RE, UK

Received December 23, 1987. Revised February 19, 1988. Accepted February 19, 1988.

Sets of overlapping cosmid clones generated by random sampling and fingerprinting methods complement data at pyrB(96.5') and oriC (84') in the published physical map of E.coli [7]. A new cloning strategy using sheared DNA, and a low copy, inducible cosmid vector were used in order to reduce bias in libraries, in conjunction with micro-methods for preparing cosmid DNA from a large number of clones. Our results are relevant to the design of the best approach to the physical mapping of large genomes.

*Present addresses: Department of Biology, University of California, San Diego, La Jolla, CA 92093, USA

+Present addresses: Institute of Biophysics, Academia Sinica, Beijing, China


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