Nucleic Acids Research, 1988, Vol. 16, No. 6 2625-2637
© 1988
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Structure and expression of the PHO80 gene of Saccharomyces cerevisiae
Department of Biological Sciences, University of Maryland Baltimore County, Catonsville, MD 21228, USA 1Department of Chemistry, Clippinger Laboratories, Ohio University Athens, OH 45701, USA
*To whom correspondance should be address
Received December 11, 1987. Accepted February 17, 1988.
In yeast, the repression of acid phosphatase under high phosphate growth conditions requires the trans-acting factor PHO80. We have determined the DNA sequence of the PHO80 gene and found that it encodes a protein of 293 amino acids. The expression of the PHO80 gene, as measured by Northern analysis and level of a PHO80-LacZ fusion protein is independent of the level of phosphate in the growth medium. Disruption of the PHO80 gene is a non-lethal event and causes a derepressed phenotype, with acid phosphatase levels which are 3-4 fold higher than the level found in derepressed wild type cells. Furthermore, over-expression of the PHO80 gene causes a reduction in the level of acid phosphatase produced under derepressed growth conditions. Finally, we have cloned, localized and sequenced a temperature-sensitive allele of PHO80 and found the phenotype to be due to T to C transition causing a substitution of a Ser for a Leu at amino acid 163 in the protein product.
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