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Nucleic Acids Research, 1988, Vol. 16, No. 7 2859-2872
© 1988

Articles

The analysls of EBV proteins which are antigenic in vivo

Dermot Walls, Michael Perricaudet1 and Frank Gannon*

Department of Microbiology, University College Galway, Ireland and BioResearch Ireland 1Institut Gustave Roussy 94800 Villejuif, France

*To whom correspondence should be addressed

Received January 11, 1988. Revised March 8, 1988. Accepted March 8, 1988.

We have used small random EBV B95-8 DNA fragments to generate a large genomic bank in a plasmid expression vector. This bank was screened with a pool of sera from individuals with IM thus allowing any EBV antigen which evoked an inmiune response in man to be identified. The characterization of four immunopositive clones obtained in this way is presented in this study. Three of these clones express viral ORF DNA sequences which are parts of larger ORFs in the BamHl N(het), V and X regions of the B95-8 viral genoma. cDNA cloning has been used to confirm that the cloned sequences from Baithil N and V are expressed in cell culture and to identify the transcription units involved. The fourth clone expresses an ORF sequence located in the viral BamHl F fragment in a region not previously recognized as having protein coding potential. The experimental design used here must reflect the situation in vivo and consequently these sequences must be expressed and be antigenic during IM.


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