Skip Navigation

This Article
Right arrow Print PDF (2465K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (101)
Right arrowRequest Permissions
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Manfioletti, G.
Right arrow Articles by Schneider, C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Manfioletti, G.
Right arrow Articles by Schneider, C.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 7 2873-2884
© 1988

Articles

A new and fast method for prearing high quality lambda DNA suitable for sequencing

Guidalberto Manfioletti and Claudio Schneider*

European Molecular Biology Laboratory Postfach 10.2209, D-6900 Heidelberg, FRG International Center for Genetic Engineering and Biotechnology Padriciano 99, 34012 Trieste, Italy

*To whom correspondence should be addressed

Received January 7, 1988. Revised March 4, 1988. Accepted March 4, 1988.

A method is described for the rapid purification of high quality lambda DNA. The method can be used from either liquid or plate lysates and on a small scale or a large scale. It relies on the preadsobtion of all polyanions present in the lysate to an "insoluble" anion-exchange matrix (DEAE or TEAE). Phage particles are then disrupted by combined treatment with EDTA/proteinase K and the resulting DNA is precipitated by the addition of the cationic detergent cetyl (or hexadecyl)-trimethyl ammonium bromide-CTAB ("soluble" anion-exchange matrix). The precipitated CTAB-DNA complex is then exchanged to Na-DNA and ethanol precipitated. The resultant purified DNA is suitable for enzymatic reactions and provides a high quality template for dideoxy-sequence analysis.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Appl. Environ. Microbiol.Home page
J. J. Gill, A. M. Svircev, R. Smith, and A. J. Castle
Bacteriophages of Erwinia amylovora
Appl. Envir. Microbiol., April 1, 2003; 69(4): 2133 - 2138.
[Abstract] [Full Text] [PDF]

Home page
 J. Bacteriol.Home page
S. Schmid, B. Berger, and D. Haas
Target Joining of Duplicated Insertion Sequence IS21 Is Assisted by IstB Protein In Vitro
J. Bacteriol., April 1, 1999; 181(7): 2286 - 2289.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
E. Carosa, A. Fanelli, S. Ulisse, R. Di Lauro, J. E. Rall, and E. A. Jannini
Ciona intestinalis nuclear receptor 1: A member of steroid/thyroid hormone receptor family
PNAS, September 15, 1998; 95(19): 11152 - 11157.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
G. K. Fu, G. Grosveld, and D. M. Markovitz
DEK, an autoantigen involved in a chromosomal translocation in acute myelogenous leukemia, binds to the HIV-2 enhancer
PNAS, March 4, 1997; 94(5): 1811 - 1815.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y.-Q. Gu, W. S. Chao, and L. L. Walling
Localization and Post-translational Processing of the Wound-induced Leucine Aminopeptidase Proteins of Tomato
J. Biol. Chem., October 18, 1996; 271(42): 25880 - 25887.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
R A Sturm, G Das, and W Herr
The ubiquitous octamer-binding protein Oct-1 contains a POU domain with a homeo box subdomain.
Genes & Dev., December 1, 1988; 2(12a): 1582 - 1599.
[Abstract] [PDF]


Disclaimer: Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.