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Nucleic Acids Research, 1988, Vol. 16, No. 7 2897-2912
© 1988

Articles

Intermediates in plasmid pT181 DNA replication

Sadhan Majumder* and Richard P. Novick

Department of Plasmid Biology, Public Health Research Institute 455 First Avenue, New York, NY 10016, USA

*To whom correspondence should be addressed at: Sloan Kettering Institute for Cancer Research, Box 46, 1275 York Avenue, New York, NY 10021, USA

Received December 2, 1987. Revised March 1, 1988. Accepted March 1, 1988.

Staphylococcus aureus plasmid pT181 is thought to replicate via an asymmetric rolling-circle mechanism. By studying pulse labeled replicative intermediates, here we report that pT181 replication involves: (1) a post-replicative hypersupercoiled monomer and (2) a partially replicated intermediate which lacks superhelicity but is unlike a typical rolling-circle intermediate in that only nascent strands of less than unit length are released by alkali accomodate this apparent discrepancy.


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