Skip Navigation

This Article
Right arrow Print PDF (3564K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Nichols, L.
Right arrow Articles by Schmidt, F. J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Nichols, L.
Right arrow Articles by Schmidt, F. J.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 7 2931-2942
© 1988

Articles

Dependence of M1 RNA substrate specificity on magnesium ion concentration

LaNita Nichols and Francis J. Schmidt*

Department of Biochemistry, University of Missouri-Columbia Columbia, MO 65212, USA

*To whom correspondence should be addressed at: Department of Biochemistry, M121 Medical Sciences Building, University of Missouri-Columbia, Columbia, MO 65212, USA

Received September 21, 1987. Revised January 25, 1988. Accepted January 25, 1988.

We have constructed a plasmid expressing E. coli M1 RNA, the catalytic RNA subunit of ribonuclease P, under the control of a phase T7 promoter. The active M1 RNA species synthesized in vitro by T7 RNA polymerase from this vector was reacted with the tRNAGln - tRNALeu precursor RNA (Band K) encoded by phage T4. Only the tRNALeu moiety of this dimeric precursor RNA contains the 3' terminal C-C-A sequence common to all tRNAs. We observed that protein-free M1 RNA was capable of processing the precursor RNA at the 5' ends of both tRNA tRNA sequences. The rate of cleavage of the tRNAGln sequence was more strongly dependent on [Mg2+] than that of tRNALeu, increasing severalfold between 100 and 500 mM Mg2+, conditions under which the rate of cleavage at the tRNA-Leu sequence was constant.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?




Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.