Nucleic Acids Research, 1988, Vol. 16, No. 8 3297-3312
© 1988
Articles |
Sequence and expression of NUC1, the gene encoding the mitochondrial nuclease in Saccharomyces cerevisiae
Department of Microbiology, St Louis University Medical Center St Louis, MO 61304, USA
*To whom correspondence should be addressed
Received December 21, 1987. Revised March 15, 1988. Accepted March 15, 1988.
The DNA sequence and studies on the expression of the NUC1 gene from Saccharomyces cerevisiae are presented. The NUC1 locus is located in the distal portion of the left arm of Chromosome X and encodes the major nuclease found in mitochondria. The inferred amino add sequence of NUC1 predicts that the nuclease is basic, rich in prolines, of average hydrophobicity, and has a molecular weight for the primary translation product of 37,209 daltons. NUC1 is very poorly expressed, consistent with the codon usage bias determined from the DNA sequence and our previous determination of the number of enzyme molecules per cell. Mapping of the 5' terminus of the NUC1 mRNA reveals that the mRNA has a long 400 base untranslated leader 1n which are found three open reading frames, each initiated by an AUG. The possibility that these upstream open reading frames contribute to the poor expression of the NUC1 gene is discussed.
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