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Nucleic Acids Research, 1988, Vol. 16, No. 8 3511-3524
© 1988

Articles

In vitro incorporation of eubacterial, archaebacterial and eukaryotic 5S rRNAs into large ribosomal subunits of Bacillus stearothermophilus

Roland K. Hartmann, Detlef W. Vogel, Richard T. Walker1 and Volker A. Erdmann

Institut für Biochemie, FB Chemie, Freie Universität Berlin Otto-Hahn-Bau, Thielallee 63, 1000 Berlin 33 (Dahlem), FRG 1Department of Chemistry, University of Birmingham Birmingham B15 2TT, UK

Received January 7, 1988. Revised March 8, 1988. Accepted March 8, 1988.

Bacillus stearothermophiluslarge ribosomal subunits were reconstituted in the presence of 5S rRNAs from different origins and tested for their biological activities. The results obtained have shown that eubacterial and archaebacterial 5S rRNAs can easily substitute for B. stearothermophilus 5S rRNA in the reconstitution, while eukaryotic 5S rRNAs yield ribosomal subunits wih reduced biological activities. From our results we propose an interaction between nucleotides 42–47 of 5S rRNA and nucleotides 2603–2608 of 23S rRNA during the assembly of the 50S ribosomal subunit. Other experiments with eukaryotic 5.8S rRNAs reveal, if at all, a very low incorporation of these RNA species into the reconstituted ribosomes.


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