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Nucleic Acids Research, 1988, Vol. 16, No. 8 3525-3543
© 1988

Articles

Synthesis and structure assignments of amide protected nucleosides and their use as phosphoramidites in deoxyoligonucleotide synthesis

Matthias Mag and Joachim W. Engels

Institut für Organische Chemie, Johann Wolfang Goethe-Universität Frankfurt Niederurseler Hang, 6000 Frankfurt am Main 50, FRG

Received January 7, 1988. Revised March 18, 1988. Accepted March 18, 1988.

The syntheses of several amide protected deoxyguanosine- as well as thymidine nucleosides are described. These compounds were synthesized according to the Mitsunobu reaction and Michael addition. In contradiction to previous studies we have discovered that the Michael addition gives only products derived from N-alkylation. The occurence of N- or O-alkylation was assigned by means of two dimensional 1H, 13C-COLOC-NMR spectroscopy. Further, we have found that the Mitsunobu reaction used for the protection of the amide function of dG is limited to alcohols without acidic hydrogen atoms. Amide protected phosphoramidites (15, 16) were used for the preparation of deoxyoligonucleotides with a large number of guanine and thymine bases using two different coupling times. We have shown that there is no experimentally detectable difference in the quality of the products if the starting monomer is amide protected or not.


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