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Nucleic Acids Research, 1988, Vol. 16, No. 9 3739-3749
© 1988

Articles

Transcriptional terminators in the caa-cal operon and cai gene

Roland Lloubès, Daniel Baty and Claude Lazdunski

Centre de Biochimie et de Biologie Moleculaire du Centre National de la Recherche Scientifique, BP 71, 31 chemin Joseph Aiguier 13402 Marseille Cedex 9, France

Received February 5, 1988. Revised April 7, 1988. Accepted April 7, 1988.

We have analysed by S1 nuclease mapping the in vivo termination sites of transcription of the caa-cal operon and cai gene. The termination region for caa mRNA (T1A terminator) features characteristics of a rho-independent terminator. This terminator is a convergent transcription terminator, its complementary secondary structure being present at the 3'-end of cai mRNA. The caa-cal mRNA terminator (T2A terminator) has a stable potential secondary structure and shows homology with rho-dependent terminators. In vitro transcription of caacal operon demonstrated that the two terminators TIA and T2A are efficient. The 3'-ends of the mRNAs which end at T1A and T2A were analysed by S1 mapping with total RNA purified from a mutant strain deficient in exoribonuclease activities, in particular RNase II. The results suggest that the potential secondary structures of T1A and T2A are sufficiently stable to prevent 3'-end degradation by RNase II. On the other hand, the T2A terminator should be efficient enough to stop transcription through the downstream DNA region involved in pColA replication.


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