Nucleic Acids Research, 1988, Vol. 16, No. 9 3751-3770
© 1988
Articles |
cDNA cloning of human hnRNP protein Al reveals the existence of multiple mRNA isoforms
Istituto di Genetica Biochimica ed Evoluzionistica, CNR, Via Abbiategrasso 207-27100 Pavia 1Istituto di Scienze Biologiche, Università degli Studi di Verona Italy
*To whom correspondence should be addressed
Received February 2, 1988. Revised March 24, 1988. Accepted March 24, 1988.
Protein Al is one of the major component of mammalian ribonucleoprotein particles (hnRNP). Human protein Al cDNA cloning and sequencing revealed the existence of at least two protein isoforms. Among the cDNAs examined, sequence differences were found both in the structural portion, leading to aminoacid changes (Tyr to Phe or Arg to Lys) and in the non +ranslated 3'-region where two T-stretches of different length were observed. Interestingly one of the aminoacid substitutions falls into a consensus sequence common to many RNA binding proteins. Northern blot analysis of poly A+ RNAs from five human tissues revealed two mRNA forms of 1500 and 1900 n due to alternative polyadenylation. Analysis of genomic DNA showed at least 30 A1-specific sequences, some of which correspond to processed pseudogenes. These results suggest that protein Al is encoded by a multigene family.
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  Y. He, M. A. Brown, J. A. Rothnagel, N. A. Saunders, and R. Smith Roles of heterogeneous nuclear ribonucleoproteins A and B in cell proliferation J. Cell Sci., July 15, 2005; 118(14): 3173 - 3183. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. Hayano, M. Yanagida, Y. Yamauchi, T. Shinkawa, T. Isobe, and N. Takahashi Proteomic Analysis of Human Nop56p-associated Pre-ribosomal Ribonucleoprotein Complexes: POSSIBLE LINK BETWEEN Nop56p AND THE NUCLEOLAR PROTEIN TREACLE RESPONSIBLE FOR TREACHER COLLINS SYNDROME J. Biol. Chem., September 5, 2003; 278(36): 34309 - 34319. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J. A. Seamon, K. S. Jones, C. Miller, and M. J. Roth Inserting a Nuclear Targeting Signal into a Replication-Competent Moloney Murine Leukemia Virus Affects Viral Export and Is Not Sufficient for Cell Cycle-Independent Infection J. Virol., July 17, 2002; 76(16): 8475 - 8484. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Sun, A. T. Alzhanova-Ericsson, N. Visa, Y. Aissouni, J. Zhao, and B. Daneholt The hrp23 Protein in the Balbiani Ring Pre-mRNP Particles Is Released Just before or at the Binding of the Particles to the Nuclear Pore Complex J. Cell Biol., September 7, 1998; 142(5): 1181 - 1193. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H.-P. Li, X. Zhang, R. Duncan, L. Comai, and M. M. C. Lai Heterogeneous nuclear ribonucleoprotein A1 binds to the transcription-regulatory region of mouse hepatitis virus RNA PNAS, September 2, 1997; 94(18): 9544 - 9549. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. Erlitzki and M. Fry Sequence-specific Binding Protein of Single-stranded and Unimolecular Quadruplex Telomeric DNA from Rat Hepatocytes J. Biol. Chem., June 20, 1997; 272(25): 15881 - 15890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. Mahe, P. Mahl, R. Gattoni, N. Fischer, M.-G. Mattei, J. Stevenin, and J.-P. Fuchs Cloning of Human 2H9 Heterogeneous Nuclear Ribonucleoproteins. RELATION WITH SPLICING AND EARLY HEAT SHOCK-INDUCED SPLICING ARREST J. Biol. Chem., January 17, 1997; 272(3): 1827 - 1836. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Inagaki, Y. Matsushima, K. Nakamura, M. Ohshima, T. Kadowaki, and Y. Kitagawa A Large DNA-binding Nuclear Protein with RNA Recognition Motif and Serine/Arginine-rich Domain J. Biol. Chem., May 24, 1996; 271(21): 12525 - 12531. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
Y. Kajita, J.-i. Nakayama, M. Aizawa, and F. Ishikawa The UUAG-specific RNA Binding Protein, Heterogeneous Nuclear Ribonucleoprotein D0 J. Biol. Chem., September 22, 1995; 270(38): 22167 - 22175. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F Weighardt, G Biamonti, and S Riva Nucleo-cytoplasmic distribution of human hnRNP proteins: a search for the targeting domains in hnRNP A1 J. Cell Sci., January 2, 1995; 108(2): 545 - 555. [Abstract] [PDF]
|
|
|
|
 |
|
 S Pinol-Roma and G Dreyfuss Transcription-dependent and transcription-independent nuclear transport of hnRNP proteins Science, July 19, 1991; 253(5017): 312 - 314. [Abstract] [PDF]
|
|
|
|
 |
|
 R J Bandziulis, M S Swanson, and G Dreyfuss RNA-binding proteins as developmental regulators. Genes & Dev., April 1, 1989; 3(4): 431 - 437. [PDF]
|
|
|
|
|
|
H. Chen, B. Hu, E. A. Allegretto, and J. S. Adams The Vitamin D Response Element-binding Protein. A NOVEL DOMINANT-NEGATIVE REGULATOR OF VITAMIN D-DIRECTED TRANSACTIVATION J. Biol. Chem., November 3, 2000; 275(45): 35557 - 35564. [Abstract] [Full Text] [PDF]
|
|