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Nucleic Acids Research, 1988, Vol. 16, No. 9 3845-3861
© 1988

Articles

Characterization of products derived from self-splicing of intron aI5{alpha} which is located in the mitochondrial COX I gene of Saccharomyces cerevisiae

A.J. Winter, G.van der Horst and H.F. Tabak

Laboratory of Biochemistry, University of Amsterdam PO Box 20151, 1000 HD Amsterdam, The Netherlands

Please address correspondence to: Mrs G.J.M.Kanis-Scholts, Section for Molecular Biology, Kruislaan 318, 1098 SM Amsterdam, The Netherlands

Received January 12, 1988. Revised March 4, 1988. Accepted March 4, 1988.

We have characterized the in vitro self-splicing of intron al5{alpha} containing precursor RNA from the yeast mitochondrial gene coding for cytochrome oxidase subunit I. This intron follows the rules for group I self-splicing introns and all the characteristic products have been identified. In addition we have detected abnormal RNA products with features that indicate that the self-splicing behaviour of this intron is more complex. Two intron circles are formed by use of a major and minor intron-internal site for circle closure. A cryptic 5'-splice site located in the 3' exon results in guanosine nucleotide mediated opening at a position 30 nt downstream of the normal 3' splice site. The reactions can all be explained on the basis of the "splice guide" model proposed by Davies et al (1982 Nature 300 719–724). Although the sequence motifs at cyclization and splice sites occur more often in this intron, only some of them are allowed to interact with the internal guide sequence, suggesting that both primary structure and spatial folding of the RNA are involved in formation of productive reaction' sites.


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