Skip Navigation

This Article
Right arrow Print PDF (1602K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Bhatt, B.
Right arrow Articles by McGee, J. OD.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Bhatt, B.
Right arrow Articles by McGee, J. OD.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 9 3951-3961
© 1988

Articles

Direct visualization of single copy genes on banded metaphase chromosomes by nonisotopic in situ hybridization

Bhupendra Bhatt, John Burns, David Flannery and James O'D. McGee

University of Oxford, Nuffield Department of Pathology, John Radcliffe Hospital Oxford OX3 9DU, UK

Received December 15, 1987. Revised March 15, 1988. Accepted March 15, 1988.

A rapid method is described for non isotopic in situ mapping of single copy genes directly on G-banded chromosomes by "one-step" regular light microscopy. It is based on hybridizing biotinylated probes to metaphase chromosomes. Biotin residues are detected by rabbit antibiotin antibody and anti-rabbit Ig labelled with peroxidase or colloidal gold. The peroxidase reaction product or colloidal gold signals are amplified by silver precipitation. The final product is a black silver dot at the gene locus on a purple G-banded chromosome. N-ras and alpha-1-antitrypsin genes have been mapped using plasmids with inserts of 1.5 and 1.3kb to 1p13.1 and the junction of 14g31/32 respectively. The signal to noise ratio in these experiments ranged from 32: 1–46: 1. This technology is at least as sensitive as radioisotopic in situ hybridization and gives results within 1 day of hybridization and has much better resolution. Additionally, genes are visualized by regular light microscopy without specialized techniques such as reflection contrast, fluorescence or phase microscopy. This methodology should facilitate more precise chromosomal gene localization.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Biol. Chem.Home page
J. Song, H. Murakami, H. Tsutsui, X. Tang, M. Matsumura, K. Itakura, I. Kanazawa, K. Sun, and K. K. Yokoyama
Genomic Organization and Expression of a Human Gene for Myc-associated Zinc Finger Protein (MAZ)
J. Biol. Chem., August 7, 1998; 273(32): 20603 - 20614.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
Y. M. Tang, Y.-Y. P. Wo, J. Stewart, A. L. Hawkins, C. A. Griffin, T. R. Sutter, and W. F. Greenlee
Isolation and Characterization of the Human Cytochrome P450 CYP1B1 Gene
J. Biol. Chem., November 8, 1996; 271(45): 28324 - 28330.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. D. Lee, K. Y. Bhakta, S. Raina, R. Yonescu, C. A. Griffin, N. G. Copeland, D. J. Gilbert, N. A. Jenkins, G. M. Preston, and P. Agre
The Human Aquaporin-5 Gene
J. Biol. Chem., April 12, 1996; 271(15): 8599 - 8604.
[Abstract] [Full Text] [PDF]

Home page
 ScienceHome page
P Lichter, C. Tang, K Call, G Hermanson, G. Evans, D Housman, and D. Ward
High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones
Science, January 5, 1990; 247(4938): 64 - 69.
[Abstract] [PDF]

Home page
 ScienceHome page
U Landegren, R Kaiser, C. Caskey, and L Hood
DNA diagnostics--molecular techniques and automation
Science, October 14, 1988; 242(4876): 229 - 237.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.