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Nucleic Acids Research, 1988, Vol. 16, No. 9 3963-3976
© 1988

Articles

Cell-specific expression of transfected brain identifier repetitive DNAs

Synthia H. Mellon+, John D. Baxter and Arthur Gutierrez-Hartmann§,*

Metabolic Research Unit and the Departments of Medicine and of Biochemistry and Biophysics, University of California San Francisco, CA 94143, USA

* To whom correspondence should be addressed

Received December 9, 1987. Revised April 8, 1988. Accepted April 8, 1988.

To define the neural-specific expression of rat repetitive identifier (ID) DNA, we co-transfected an intron B subclone of the rat growth hormone (rGH) gene, containing a tandem array of two type 2 repeats and a single ID monomer, and a plasmid conferring neomycin resistance into human SK-N-MC neuroblastoma, HeLa epidermal carcinoma, 293 kidney and 251 MG glioblastoma cells. Transcript analysis from both individual and pools of G418-resistant cells revealed that rGH intron B repeats were expressed only in SK-N-MC neuroblastoma cells as small, cytoplasmic RNAs of 85, 110, 155 and 180 bases. Primer-extension studies show these repetitive RNAs to contain a common 5' end that maps precisely to the beginning of the ID element and that type 2 transcripts are not stably expressed. However, ID DNA expression from two other transfected plasmids, each containing only the ID core sequence, was not restricted to the SK-N-MC cell line. These data show that the transfected rGH ID sequence is selectively expressed in a neural-specific manner resulting in BC-like RNAs, and furthermore, suggest that flanking DNA may play a role in cell-specific expression of certain repetitive DNA elements.

+ Present addresses: Department of Obstetrics, Gynecology and Reproductive Sciences, Box 0546, University of California, San Francisco, CA 94143

§Departments of Medicine and of Biochemistry, Biophysics and Genetics, B-151, University of Colorado Health Sciences Center, Denver, CO 80262, USA


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