Skip Navigation

This Article
Right arrow Print PDF (856K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (50)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Hatt, C.
Right arrow Articles by Clarke, I. N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hatt, C.
Right arrow Articles by Clarke, I. N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1988, Vol. 16, No. 9 4053-4067
© 1988

Articles

Analysis of the entire nucleotide sequence of the cryptic plasmid of Chlamydia trachomatis serovar L1. Evidence for involvement in DNA replication

Christopher Hatt, Michael E. Ward and Ian N. Clarke

Department of Microbiology, Faculty of Medicine (University of Southampton) South Academic Pathology Block, Southampton General Hospital Tremona Road, Southampton, SO9 4XY, UK

Received February 12, 1988. Revised March 15, 1988. Accepted March 15, 1988.

Chlamydia trachomatis serovar L1/440/LN possesses a 7498bp plasmid which was designated pLGV440. The plasmid was cloned at the BamHl site of pAT153 into Escherichia coli and the recombinant plasmid was designated pCTL1. A detailed restriction endonuclease map of pCTL1 was constructed. A fragment of the chlamydial plasmid was shown to function as a promoter in E. coli when placed upstream of the lacZ gene. The entire plasmid was sequenced by the chain termination method. Open reading frames were identified from the resulting consensus sequence together with a candidate for the plasmid origin of replication consisting of four perfect tandem repeats of a 22bp sequence, an A: T rich sequence and an open reading frame which could generate a 34.Bkdal product. The predicted polypeptide products of the open reading frames were compared by computer with all reported protein sequences. Homology of the predicted polypeptide product of an open reading frame to the E. coli dnaB protein and the analogous product of gene 12 of bacteriophage P22 is described.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Infect. Immun.Home page
Z. Li, D. Chen, Y. Zhong, S. Wang, and G. Zhong
The Chlamydial Plasmid-Encoded Protein pgp3 Is Secreted into the Cytosol of Chlamydia-Infected Cells
Infect. Immun., August 1, 2008; 76(8): 3415 - 3428.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
P. R. Lambden, M. A. Pickett, and I. N. Clarke
The effect of penicillin on Chlamydia trachomatis DNA replication.
Microbiology, September 1, 2006; 152(Pt 9): 2573 - 2578.
[Abstract] [Full Text] [PDF]

Home page
 MicrobiologyHome page
M. A. Pickett, J. S. Everson, P. J. Pead, and I. N. Clarke
The plasmids of Chlamydia trachomatis and Chlamydophila pneumoniae (N16): accurate determination of copy number and the paradoxical effect of plasmid-curing agents
Microbiology, March 1, 2005; 151(3): 893 - 903.
[Abstract] [Full Text] [PDF]

Home page
 Antimicrob. Agents Chemother.Home page
J. Lenart, A. A. Andersen, and D. D. Rockey
Growth and Development of Tetracycline-Resistant Chlamydia suis
Antimicrob. Agents Chemother., August 1, 2001; 45(8): 2198 - 2203.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Microbiol.Home page
A. Matsumoto, H. Izutsu, N. Miyashita, and M. Ohuchi
Plaque Formation by and Plaque Cloning of Chlamydia trachomatis Biovar Trachoma
J. Clin. Microbiol., October 1, 1998; 36(10): 3013 - 3019.
[Abstract] [Full Text]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.