Nucleic Acids Research, 1989, Vol. 17, No. 1 107-117
© 1989
MOLECULAR BIOLOGY |
Trans-activation by the c-myb proto-oncogene
1Laboratory of Molecular Genetics, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN) 3-1-1 Koya, Tsukuba, Ibaraki 305, Japan 2Frontier Research Programs, Tsukuba Life Science Center, Institute of Physical and Chemical Research (RIKEN) 3-1-1 Koya-dai, Tsukuba, Ibaraki 305 3Department of Pharmacy, Science University of Tokyo Funagawara-cho 12, Ichigaya, Shinjuku-ku 162, Japan 4Ludwig Institute for Cancer Research, Melbourne Tumour Biology Branch, PO Royal Melbourne Hospital Victoria 3050, Australia
Received October 13, 1988. Revised December 5, 1988. Accepted December 5, 1988.
We present evidence that the mouse c-myb proto-oncogene encodes a transcriptional trans-activator. Trans-activation was assayed by cotransfection into CV1 monkey kidney cells of a c-myb cDNA exprssion plasmid together with a reporter plasmid carrying the chloramphenocol acetyltransferase (CAT) gene under the control of a test promoter and enhancer. Cotransfection with the c-myb cDNA plasmid caused a 20-fold stimulation of transcription from the promoter of the mouse 
2(I) collagen gene linked to tandem repeats of the simian virus 40 (SV40) enhancer element. Using different promoters in combination with varying numbers of repeats of the SV40 enhancer element, it was shown that tandem repeats of the SV40 enhancer mediated the c-myb-induced activation of transcription. These results show that the mouse c-myb gene product either is itself or induces, an activator of transcription that recognizes specific sequences in the SV40 enhancer.
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