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Nucleic Acids Research, 1989, Vol. 17, No. 1 119-133
© 1989

MOLECULAR BIOLOGY

Different liver nuclear proteins bind to similar DNA sequences in the 5' flanking regions of three hepatic genes

Alberto Ochoa+, Franck Brunel§, Daniel Mendelzon*, Georges N. Cohen and Mario M. Zakin*

Unité de Biochimie Cellulaire, Institut Pasteur 28 rue du Docteur Roux, 75724 Paris Cedex 15, France +Pre-doctoral fellow of the Association pour la Recherche sur le Cancer France §Pre-doctoral fellow of the Ministère de la Recherche et de I'Enseignement Supérieur France *Post-doctoral fellow of the Consejo Nacional de Investigaciones Cientificas y Técnicas Argentina

*To whom correspondence should be addressed

Received October 13, 1988. Accepted December 2, 1988.

The proximal promoter region of the human transferrin gene contains an hepatocyte-specific cis-element (PRI, nucleotides –76 to –51) whose DNA sequence is homologous to a sequence (nucleotides –89 to –68) present in the transcriptionally essential 5' region of the human antithrombin III gene and to another hepatocyte-specific sequence (A domain) of the human {alpha}1-antitrypsin gene promoter. The results reported here lead to the conclusion that the liver trans-acting factor Tf-LF1, binding to the transferrin PRI cis-element interacts with the homologous antithrombin III region, but is different from the transcription factor LF-A1 interacting with the A domain of the {alpha}1-antitrypsin promoter.

The distal region DRI (nucleotides –480 to –454) of the human transferrin gene promoter presents in its core the same 10 nucleotide-long sequence as the PRI cis-element. We have previously shown that the liver protein Tf-LF2, binding to the DRI element is different from the Tf-LF1 trans-acting factor. In this paper we also show that Tf-LF2 is different from the transcription factor LF-Al interacting with the {alpha}1-antitrypsin promoter.

The results allow us to conclude that at least three distinct liver nuclear proteins bind to different subsets of 5' DNA regions containing similar sequences. These sequences are present in genes expressed essentially in liver.


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