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Nucleic Acids Research, 1989, Vol. 17, No. 1 163-170
© 1989

MOLECULAR BIOLOGY

Isolation of an IgH gene circular DNA clone from human bone marrow

Masumi Abe and Hiroshi Shiku*

Department of Oncology, Nagasaki University School of Medicine 12-4 Sakamoto-machi, Nagasaki 852, Japan

* To whom correspondence should be addressed.

Received October 10, 1988. Revised November 23, 1988. Accepted November 23, 1988.

Circular DNA was obtained from human bone marrow. Then a phage library was prepared and screened by use of two probes of the IgH gene; 5'-DHQ52, containing the 5' flanking region of DHQ52, and JH4.3, containing the sequence from JH3 to the 3' flanking region of JH6. One clone, HBMC-1, that was DHQ52+JH4.3– was obtained. HBMC-1 had the germline IgH region upstream of JH1 and the 3' flanking region of DXP1. A recombination signal sequence flanking the 5' side of the JH1 segment was attached to the recombination signal sequence flanking the 3' side of DXP1 forming a head-to-head structure of two 7mers with 10 nucleotides in-between. HBMC-1 is thus considered to be a circular DNA deleted as a consequence of DXP1-JH1 joining of the IgH gene.


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