Skip Navigation

This Article
Right arrow Print PDF (547K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (91)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Shibahara, S.
Right arrow Articles by Yamamoto, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Shibahara, S.
Right arrow Articles by Yamamoto, N.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 1 239-252
© 1989

CHEMISTRY

Inhibition of human immunodeficiency virus (HIV-1) replication by synthetic oligo-RNA derivatives

Susumu Shibahara, Sachiko Mukai, Hirokazu Morisawa, Hideki Nakashima1, Susumu Kobayashi1 and Naoki Yamamoto1

Central Research Laboratories, Ajinomoto Co., Inc. Suzuki-cho, Kawasaki 210 1Department of Virology and Parasitology, Yamaguchi University School of Medicine 1144 Kogushi, Ube, Yamaguchi 755, Japan

Received September 2, 1988. Revised November 30, 1988. Accepted November 30, 1988.

Several synthetic 2'-0-methyl-RNA oligomers and their derivatives have been evaluated for inhibitory effect against HIV-induced cytopathic effect and expression of the virus specific antigen in cultured MT-4 cells. In this study, oligo(2'-0-methyl)ribonucleoside phosphorothioates showed a potent inhibitory activity with size dependency (25-mer showed it at 1 uM), but by contrast both 2'-0-methylribo- and deoxy-oligomers with normal phosphate linkages failed to inhibit. However, it should be noted that the patched oligo(2'-0-methyl)ribonucleotide (20-mer), in which five linkages at 5'and three linkages at 3'-ends of normal phosphates were replaced with thiophosphates, has recovered the substantial inhibitory effect. These results show that the size of oligomer and phosphorothioate linkages, probably resistant to exolytic nucleases, are essential for exhibiting antiviral activity.


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Ann. N. Y. Acad. Sci.Home page
G. IVANOVA, A. A ARZUMANOV, J. J TURNER, S. REIGADAS, J.-J. TOULME, D. E BROWN, A. M.L LEVER, and M. J GAIT
Anti-HIV Activity of Steric Block Oligonucleotides
Ann. N.Y. Acad. Sci., October 1, 2006; 1082(1): 103 - 115.
[Abstract] [Full Text] [PDF]

Home page
 Ann. N. Y. Acad. Sci.Home page
Y. S. CHO-CHUNG, M. NESTEROVA, K. G. BECKER, R. SRIVASTAVA, Y. G. PARK, Y. N. LEE, Y. S. CHO, M.-K. KIM, C. NEARY, and C. CHEADLE
Dissecting the Circuitry of Protein Kinase A and cAMP Signaling in Cancer Genesis: Antisense, Microarray, Gene Overexpression, and Transcription Factor Decoy
Ann. N.Y. Acad. Sci., June 1, 2002; 968(1): 22 - 36.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Scherr, M. Grez, A. Ganser, and J. W. Engels
Specific Hammerhead Ribozyme-mediated Cleavage of Mutant N-ras mRNA in Vitro and ex Vivo. OLIGORIBONUCLEOTIDES AS THERAPEUTIC AGENTS
J. Biol. Chem., May 30, 1997; 272(22): 14304 - 14313.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.