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Nucleic Acids Research, 1989, Vol. 17, No. 1 271-284
© 1989

MOLECULAR BIOLOGY

A 12 megabase restriction map at the cystic fibrosis locus

T.R. Fulton, A.M. Bowcock1, D.R. Smith*, L. Daneshvar1, P. Green, L.L. Cavalli-Sforza1 and H. Donis-Keller

Department of Human Genetics, Collaborative Research Inc. Bedford, MA 1Department of Genetics, Stanford University Stanford, CA, USA

*To whom correspondence should be addressed

Received August 26, 1988. Revised November 21, 1988. Accepted November 21, 1988.

We have constructed a physical map of the chromosomal region containing the cystic fibrosis locus using seven DNA markers and pulsed-field gel electrophoresis methods. The map includes cleavage sites for 8 rare-cutting restriction enzymes and spans over 12 megabases (Mb) of DNA, with one unlinked probe covering an additional 5 Mb. To our knowledge, this is the largest segment of human DNA which has been restriction mapped to date. We can identify thirteen putative HTF islands spaced at intervals of 0.3 – 3.2 Mb. The region between loci D7S8 and MET, where the CF gene lies, includes 1.4 – 1.9 Mb of DNA.


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