Skip Navigation

This Article
Right arrow Print PDF (1537K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (19)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Trist, H.
Right arrow Articles by Phillips, D. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Trist, H.
Right arrow Articles by Phillips, D. R.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 10 3673-3688
© 1989

MOLECULAR BIOLOGY

In vitro transcription analysis of the role of flanking sequence on the DNA sequence specificity of Adriamycin

Halina Trist and Don R. Phillips*

Department of Biochemistry, La Trobe University Bundoora, Victoria 3083, Australia

*To whom correspondence should be addressed

Received March 2, 1989. Revised April 12, 1989. Accepted April 12, 1989.

An in vitro transcription assay has been used to define twelve high occupancy transcriptional blockage sites in 260 bp of heterogenous DNA on three promoter-containing DNA fragments. Transcription proceeded to immediately upstream of CpA sequences in nine of these sites, and this defines the most preferred intercalation site as CpA. In almost all cases, this sequence was flanked by T on the 5' end. The consensus sequence for the highest affinity Adriamycin site is therefore 5'-TCA, with some evidence for preference of AT base pairs flanking both ends of this trinucleotide [i.e.,(t)TCA(a.t)(a.t)]. In contrast, lower occupancy CpA sites were flanked on the 5' end by a GC base pair, with a preference for up to three base pairs on the 3' end. The low affinity consensus sequence is therefore (G.C)CA(g.c)(g.c)(g.c).


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Pharmacol.Home page
B. J. Evison, F. Chiu, G. Pezzoni, D. R. Phillips, and S. M. Cutts
Formaldehyde-Activated Pixantrone Is a Monofunctional DNA Alkylator That Binds Selectively to CpG and CpA Doublets
Mol. Pharmacol., July 1, 2008; 74(1): 184 - 194.
[Abstract] [Full Text] [PDF]

Home page
 Nucleic Acids ResHome page
D. J. Cashman, J. N. Scarsdale, and G. E. Kellogg
Hydropathic analysis of the free energy differences in anthracycline antibiotic binding to DNA
Nucleic Acids Res., August 1, 2003; 31(15): 4410 - 4416.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.