Skip Navigation

This Article
Right arrow Print PDF (4617K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (64)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Lasker, B.A.
Right arrow Articles by Medoff, G.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Lasker, B.A.
Right arrow Articles by Medoff, G.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 10 3783-3793
© 1989

CHEMISTRY

Comparison of the separation of Candida albicans chromosome-sized DNA by pulsed-field gel electrophoresis techniques

B.A. Lasker*, G.F. Carle+, G.S. Kobayashi and G. Medoff

Divisions of Infectious Diseases, of Dermatology, and of Laboratory Medicine, Department of Medicine and Department of Genetics Washington University School of Medicine, St Louis, MO 63110, USA

Received November 16, 1988. Revised April 12, 1989. Accepted April 12, 1989.

Pulsed-field gel electrophoresis techniques were used to study chromosome-sized DNA molecules of C. albicans. Chromosome-sized DNA of two strains of Candida albicans has been resolved into 8 bands by orthogonal-field-alternation gel electrophoresis (OFAGE). Six bands were observed in chromosomal preparations of C. albicans using field-inversion gel electrophoresis (FIGE). Differences in the electrophoretic mobilities of bands of the strains of C. albicans examined suggests that chromosome-length polymorphisms exist and make it difficult to correlate the banding patterns among strains. These correlations were facilitated, however, by assignment of C. albicans chromosomes by hybridization using a collection of cloned DNA probes specific for each of the 8 observed bands. Southern blotting showed that the 6 FIGE bands consisted of 4 singlets and 2 comigrating doublets, accounting for the 8 bands observed by OFAGE analysis. The agreement between OFAGE and FIGE analysis suggests that the C. albicans haploid genome contains a minimum of 8 chromosomes.

*Present addresses BAL. Mycology Section. Center for Disease Control. Atlanta, GA 30333. USA

+Present addresses GFC, Centre de Biochimie. Université de Nice. 06034 Nice Cedex. France


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 J. Clin. Microbiol.Home page
Y. H. Samaranayake, L. P. Samaranayake, E. H. N. Pow, V. T. Beena, and K. W. S. Yeung
Antifungal Effects of Lysozyme and Lactoferrin against Genetically Similar, Sequential Candida albicans Isolates from a Human Immunodeficiency Virus-Infected Southern Chinese Cohort
J. Clin. Microbiol., September 1, 2001; 39(9): 3296 - 3302.
[Abstract] [Full Text] [PDF]

Home page
 Genome Res.Home page
H. Chibana, J. L. Beckerman, and P.T. Magee
Fine-Resolution Physical Mapping of Genomic Diversity in Candida albicans
Genome Res., December 1, 2000; 10(12): 1865 - 1877.
[Abstract] [Full Text]

Home page
 J. Clin. Microbiol.Home page
W. K. Leung, R. S. Dassanayake, J. Y. Y. Yau, L. J. Jin, W. C. Yam, and L. P. Samaranayake
Oral Colonization, Phenotypic, and Genotypic Profiles of Candida Species in Irradiated, Dentate, Xerostomic Nasopharyngeal Carcinoma Survivors
J. Clin. Microbiol., June 1, 2000; 38(6): 2219 - 2226.
[Abstract] [Full Text]

Home page
 Clin. Microbiol. Rev.Home page
D. R. Soll
The Ins and Outs of DNA Fingerprinting the Infectious Fungi
Clin. Microbiol. Rev., April 1, 2000; 13(2): 332 - 370.
[Abstract] [Full Text] [PDF]

Home page
 NEJMHome page
S. N. Bennett, M. M. McNeil, L. A. Bland, M. J. Arduino, M. E. Villarino, D. M. Perrotta, D. R. Burwen, S. F. Welbel, D. A. Pegues, L. Stroud, et al.
Postoperative Infections Traced to Contamination of an Intravenous Anesthetic, Propofol
N. Engl. J. Med., July 20, 1995; 333(3): 147 - 154.
[Abstract] [Full Text] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.