Skip Navigation

This Article
Right arrow Print PDF (1362K)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Add to My Personal Archive
Right arrow Download to citation manager
Right arrow Search for citing articles in:
ISI Web of Science (56)
Right arrow Commercial Re-use Guidelines
for Open Access NAR Content
Google Scholar
Right arrow Articles by Goldstein, S.
Right arrow Articles by Howard, B. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Goldstein, S.
Right arrow Articles by Howard, B. H.
Social Bookmarking
Add to CiteULike   Add to Connotea   Add to Del.icio.us  
What's this?

Nucleic Acids Research, 1989, Vol. 17, No. 10 3959-3971
© 1989

CHEMISTRY

Enhanced transfection efficiency and improved cell survival after electroporation of G2/M-synchronized cells and treatment with sodium butyrate

Samuel Goldstein*,+, C.Michael Fordis and Bruce H. Howard

Laboratory of Molecular Biology, National Cancer Institute, National Institutes of Health Bethesda, MD 20892, USA

*To whom correspondence should be addressed

Received January 10, 1989. Revised April 12, 1989. Accepted April 12, 1989.

To achieve high transfection efficiency in human fibroblasts with good preservation of proiiferative capacity we developed an electroporation procedure that combines two distinct modalities: use of recipient cells synchronized in the late G2/mitotic phase of the cell cycle and treatment of cells post-electroporation with 5 mM butyrate. This combination enabled reduction of plasmid DNA concentration and electroporation voltage, both associated with cytotoxicity, while greatly enhancing transfection efficiencies. Although the method was primarily developed for transient expression it was also found to improve stable expression. This procedure should have wide applicability, particularly in studies seeking to identify DNA sequences that lead to inhibition of DNA synthesis and proliferation in human fibroblasts and other cells refractory to transfection.

+Present address: Departments of Medicine, Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences and Geriatnc Research Education and Clinical Center, John L.McClellan Memorial Veterans Hospital, 151 Research, Little Rock, AR 72205, USA


Add to CiteULike CiteULike   Add to Connotea Connotea   Add to Del.icio.us Del.icio.us    What's this?


This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
R. V. Intine, M. Dundr, A. Vassilev, E. Schwartz, Y. Zhao, Y. Zhao, M. L. DePamphilis, and R. J. Maraia
Nonphosphorylated Human La Antigen Interacts with Nucleolin at Nucleolar Sites Involved in rRNA Biogenesis
Mol. Cell. Biol., December 15, 2004; 24(24): 10894 - 10904.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Biol.Home page
A. Katsumi, J. Milanini, W. B. Kiosses, M. A. del Pozo, R. Kaunas, S. Chien, K. M. Hahn, and M. A. Schwartz
Effects of cell tension on the small GTPase Rac
J. Cell Biol., July 8, 2002; 158(1): 153 - 164.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
P. R. Kiela, E. R. Hines, J. F. Collins, and F. K. Ghishan
Regulation of the rat NHE3 gene promoter by sodium butyrate
Am J Physiol Gastrointest Liver Physiol, October 1, 2001; 281(4): G947 - G956.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
G. Ranganathan, D. Vu, and P. A. Kern
Translational Regulation of Lipoprotein Lipase by Epinephrine Involves a Trans-acting Binding Protein Interacting with the 3' Untranslated Region
J. Biol. Chem., January 24, 1997; 272(4): 2515 - 2519.
[Abstract] [Full Text] [PDF]

Home page
 J. Cell Sci.Home page
D. Grant, J. Kinsella, M. Kibbey, S LaFlamme, P. Burbelo, A. Goldstein, and H. Kleinman
Matrigel induces thymosin beta 4 gene in differentiating endothelial cells
J. Cell Sci., January 12, 1995; 108(12): 3685 - 3694.
[Abstract] [PDF]


Disclaimer:
Please note that abstracts for content published before 1996 were created through digital scanning and may therefore not exactly replicate the text of the original print issues. All efforts have been made to ensure accuracy, but the Publisher will not be held responsible for any remaining inaccuracies. If you require any further clarification, please contact our Customer Services Department.