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Nucleic Acids Research, 1989, Vol. 17, No. 11 4037-4045
© 1989

MOLECULAR BIOLOGY

The mouse homologue of hst/ k-FGF: sequence, genome organization and location relative to int-2

Sharon Brookes, Rosalind Smith1, Jan Thurlow1, Clive Dickson1 and Gordon Peters

1imperial Cancer Research Fund Laboratories PO Box 123, Lincoln's Inn Fields, London WC2A 3PX, UK Imperial Cancer Research Fund Laboratories, St Bartholomew's Hospital Dominion House, Bartholomew Close, London EC1A 7BE

Received March 30, 1989. Accepted May 3, 1989.

We have located and sequenced the murine homologue of the hst/k-FGF oncogene in genomic DNA clones that extend 3' from int-2 on mouse chromosome 7. The two genes are in the same transcnptional orientation, less than 20 kilobase pairs (kb) apart, and presumably evolved by tandem duplication of a common ancestral gene. RNase mapping and primer extension analyses indicated that the major 3.2 kb hst transcript expressed in undifferentiated embryonal carcinoma (EC) cell lines initiates at a unique cap site downstream from an obvious TATA-box. The 3' end of the transcript as identified in multiple cDNA clones occurs at an appropriate distance from a variant polyadenylation signal, ATTAAA. Translation of the major open reading frame would yield a 202 arnino acid protein that is 82% homologous to human HST but lacking 4 residues at the presumed signal peptide cleavage site.


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