Nucleic Acids Research, 1989, Vol. 17, No. 11 4145-4159
© 1989
ENZYMOLOGY |
ATPase activity of the UvrA and UvrAB protein complexes of the Escherichia coli UvrABC endonuclease
The Johns Hopkins University, Department of Biochemistry, School of Hygiene and Public Health 615 North Wolfe Street, Baltimore, MD 21205, USA
*To whom correspondence should be addressed
Received November 30, 1988. Revised May 5, 1989. Accepted May 5, 1989.
We have analyzed the ATPase activity exhibited by the UvrABC DNA repair complex. The UvrA protein is an ATPase whose lack of DNA dependence may be related to the ATP induced monomerdimer transitions. ATP induced dimerization may be responsible for the enhanced DNA binding activity observed in the presence of ATP. Although the UvrA ATPase is not stimulated by dsDNA, such DNA can modulate the UvrA ATPase activity by decreases in Km and Vm and alterations in the K1 for ADP and ATP-
-S. The induction of such changes upon binding to DNA may be necessary for cooperative interactions of UvrA with UvrB that result in a DNA stimulated ATPase for the UvrAB protein complex. The UvrAB ATPase displays unique kinetic profiles that are dependent on the structure of the DNA effector. These kinetic changes correlate with changes in footprinting patterns, the stabilization of protein complexes on DNA damage and with the expression of helicase activity.
+Present address: Department of Biochemistry and Biophysics, University of California at San Francisco, San Francisco, CA, USA
CiteULike 
Connotea 
Del.icio.us What's this?
This article has been cited by other articles:
|
|
 |
|
  K. Wagner, G. Moolenaar, J. van Noort, and N. Goosen Single-molecule analysis reveals two separate DNA-binding domains in the Escherichia coli UvrA dimer Nucleic Acids Res., April 1, 2009; 37(6): 1962 - 1972. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 I. Gomez-Pinto, E. Cubero, S. G. Kalko, V. Monaco, G. van der Marel, J. H. van Boom, M. Orozco, and C. Gonzalez Effect of Bulky Lesions on DNA: SOLUTION STRUCTURE OF A DNA DUPLEX CONTAINING A CHOLESTEROL ADDUCT J. Biol. Chem., June 4, 2004; 279(23): 24552 - 24560. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Yamagata, R. Masui, R. Kato, N. Nakagawa, H. Ozaki, H. Sawai, S. Kuramitsu, and K. Fukuyama Interaction of UvrA and UvrB Proteins with a Fluorescent Single-stranded DNA. IMPLICATION FOR SLOW CONFORMATIONAL CHANGE UPON INTERACTION OF UvrB WITH DNA J. Biol. Chem., April 28, 2000; 275(18): 13235 - 13242. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. F. Moolenaar, V. Monaco, G. A. van der Marel, J. H. van Boom, R. Visse, and N. Goosen The Effect of the DNA Flanking the Lesion on Formation of the UvrB-DNA Preincision Complex. MECHANISM FOR THE UvrA-MEDIATED LOADING OF UvrB ONTO A DNA DAMAGED SITE J. Biol. Chem., March 10, 2000; 275(11): 8038 - 8043. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Machius, L. Henry, M. Palnitkar, and J. Deisenhofer Crystal structure of the DNA nucleotide excision repair enzyme UvrB from Thermus thermophilus PNAS, October 12, 1999; 96(21): 11717 - 11722. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Hildebrand and L. Grossman Oligomerization of the UvrB Nucleotide Excision Repair Protein of Escherichia coli J. Biol. Chem., September 24, 1999; 274(39): 27885 - 27890. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. I. Kovalsky and L. Grossman Accessibility of Epitopes on UvrB Protein in Intermediates Generated during Incision of UV-irradiated DNA by the Escherichia coli Uvr(A)BC Endonuclease J. Biol. Chem., August 14, 1998; 273(33): 21009 - 21014. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. L. Hildebrand and L. Grossman Introduction of a Tryptophan Reporter Group into the ATP Binding Motif of the Escherichia coli UvrB Protein for the Study of Nucleotide Binding and Conformational Dynamics J. Biol. Chem., April 3, 1998; 273(14): 7818 - 7827. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Nakagawa, R. Masui, R. Kato, and S. Kuramitsu Domain Structure of Thermus thermophilus UvrB Protein. SIMILARITY IN DOMAIN STRUCTURE TO A HELICASE J. Biol. Chem., September 5, 1997; 272(36): 22703 - 22713. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
O. I. Kovalsky, L. Grossman, and B. Ahn The Topodynamics of Incision of UV-irradiated Covalently Closed DNA by the Escherichia coli Uvr(A)BC Endonuclease J. Biol. Chem., December 27, 1996; 271(52): 33236 - 33241. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ahn and L. Grossman RNA Polymerase Signals UvrAB Landing Sites J. Biol. Chem., August 30, 1996; 271(35): 21453 - 21461. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Ahn and L. Grossman The Binding of UvrAB Proteins to Bubble and Loop Regions in Duplex DNA J. Biol. Chem., August 30, 1996; 271(35): 21462 - 21470. [Abstract] [Full Text] [PDF]
|
|