Nucleic Acids Research, 1989, Vol. 17, No. 11 4353-4357
© 1989
CHEMISTRY |
Automated polymerase chain reaction in capillary tubes with hot air
Department of Pathology, University of Utah Medical School Salt Lake City, UT 84132, USA
*To whom correspondence should be addressed.
Received January 31, 1989. Revised April 10, 1989. Accepted April 10, 1989.
We describe a simple, compact, inexpensive thermal cycler that can be used for the polymerase chain reaction. Based on heat transfer with air to samples in sealed capillary tubes, the apparatus resembles a recirculating hair dryer. The temperature is regulated via thermocouple input to a programmable set-point process controller that provides proportional output to a solid state relay controlling a heating coil. For efficient cooling after the denaturation step, the controller activates a solenoid that opens a door to vent hot air and allows cool air to enter. Temperature-time profiles and amplification results approximate those obtained using water baths and microfuge tubes.
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